Pancreatic Digest Chocolate Blood Agar for the Isolation of the Gonococcus.
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Neisseria species are gram negative diplococci an important characteristic of these bacteria is resistance against crystal violet. This study was done to investigate the effect of crystal violet on the growth of Neisseria, to observe the outcome of adding this substance in culture media for isolating these organisms, and finally to make a specific medium for isolation of the Nisseria species. ...
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The incorporation of trimethoprim into existing selective media using either saponin-lysed blood agar or chocolate agar as the base is suggested for the routine isolation of the gonococcus. The principal advantage over other selective media is the inhibition of Proteus mirabilis and other commensal organisms as a consequence of which multiple swabs can be plated out onto a single selective plat...
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Media for isolation of Haemophilus sp. from the conjuctiva were compared in an oasis in southern Tunisia where severe trachoma and seasonal epidemic purulent conjunctivitis are common. Of 89 children tested, IsoVitaleX-supplemented chocolate agar yielded Haemophilus in 87%, plain chocolate agar in 75%, sheep blood agar with a stab of Staphylococcus epidermidis in 74%, and Fildes medium in 58%. ...
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A culture medium that is selective for the gonococcus and the meningococcus has been described recently (Thayer and Martin, 1964). The medium with Polymyxin and Ristocetin added permits isolation of the gonococcus from sites highly contaminated with other bacteria. Overgrowth of gonococcal colonies by the commensal bacterial flora present in rectal, vaginal, and cervical specimens is almost com...
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A useful method for isolating and recognizing Haemophilus ducreyi from chancres and buboes of male patients is presented. A total of 41 clinical isolates of H. ducreyi were recovered from 33 patients over an 8-year period, and the experience with the 15 most recent isolates is presented in detail. Chocolate agar supplemented with 1% Iso VitaleX and 5% sheep blood agar were prepared, using Trypt...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 56 1 شماره
صفحات -
تاریخ انتشار 1948